The Effect of Adding Lactic Acid Bacteria Type Pediococcus pentosaceus on Phosphorus Content of Rice Bran

Authors

  • Zaid Al Gifari Fakultas Peternakan Universitas Mataram
  • Rina Andriati Program Studi S1 Peternakan, Fakultas Peternakan, Universitas Mataram, Mataram, Indonesia
  • Muhamad Ali Fakultas Peternakan Universitas Mataram
  • Khairil anwar Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa Ward, Nagoya City, Aichi 464-8601

DOI:

https://doi.org/10.29303/jitpi.v11i1.222

Keywords:

Pediococcus pentosaceus, Rice bran, Phosphorus, Fermentation, Probiotics

Abstract

This study aims to evaluate the effect of fermentation with lactic acid bacteria Pediococcus pentosaceus on phosphorus content in rice bran. This bacteria is a probiotic isolated from animal intestinal microflora, with high colonization ability due to its suitability to the original environment. The study focused on the ability of the isolate to break down phytic acid bound to the bran, with the main indicator being an increase in phosphorus levels. The fermentation results showed the highest viability at an incubation temperature of 37°C, with a maximum number of 240 × 10⁸ CFU/g at 12 hours, but decreased thereafter. Although there was an increase in phosphorus levels, the results were not statistically significant (p>0.05) in both dry bran (P1) and wet bran (P2) treatments. The highest increase in phosphorus occurred at a temperature of 44°C (0.91±0.004%; +25%) and at 37°C (0.87±0.002%; +19%). This suggests that temperature affects the effectiveness of fermentation, but water content and incubation time may be limiting factors. Overall, fermentation of rice bran with P. pentosaceus did not show a significant increase in phosphorus levels.

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References

Akande, Akubakar, Adegbola, Bogoro, and Doma. (2010). Chemical (Cajanus cajan). Int. J. of Poultry Science, 9,(1).

Aritonang, S. N., Roza, E., Rossi, E., Purwati, E., and Husmaini, H. (2017). Isolation and Identification of Lactic Acid Bacteria from Okara and Evaluation of Their Potential as Candidate Probiotics. Pakistan J. Nutri, 16, 618-628.

Bohn, L., Meyer, A. S., and Rasmussen, S. K. (2008). Phytate: Impact on Environment and Human Nutrition A Challenge for Molecular Breeding. J Zhejiang Univ Sci B, 9, 165-191.

Dwidjoseputro, D. (2005). Dasar-Dasar Mikrobiologi. Djambatan.

Fauziah, R. N., Sari, N. A., and Nurbaety, A. T. (2011). Pengaruh Suhu dan Salinitas terhadap Viabilitas Bakteri Aeromonas hydrophila dan Bacillus sp. Departemen Budidaya Perairan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.

Gifari, Z. A., Agistna, I., Anwar, K., Rosyidi, A., Ali, M., and Amin, M. (2022). Cultivation of Phytase-Producing Bacteria as Probiotic Candidate on Molasses and Tempe-Processing Waste. IOP Conference Series: Earth and Environmental Science,1036,(1),012048. https://doi.org/10.1088/1755-1315/1036/1/012048

Gifari, Z. A., Anwar, K., Rosyidi, A., Ali, M., and Amin, M. (2022). Isolasi Dan Identifikasi Bakteri Asam Laktat Penghasil Enzim Fitase Sebagai Kandidat Probiotik Untuk Ternak Unggas. Syntax Literate: Jurnal Ilmiah Indonesia, 7,(6), 2541-0849.

Gifari, Z. A., Khairunnisah, Bagis, F. A. Z., Andriani, F., Anwar, K., Suryadi, M. A. F., Alimuddin, Amin, M., Rosyidi, A., Wariata, I. W., Sriasih, M., and Ali, M. (2024). Comparison of Extracellulase-producing Bacillus Isolated from the Digestive Tract of Muscovy Ducks (Cairina Moschata) and Native Chicken (Gallus Gallus Domesticus) as Probiotic Candidates for Poultry. in Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024), Advances in Biological Sciences Research 45. https://doi.org/10.2991/978-94-6463-670-3_36

Haddadin, M. S. Y., Haddadin, J., Arabiyat, O. I., and Hattar, B. (2009). Biological conversion of olive pomace into compost by using Trichoderma harzianum and Phanerochaete chrysosporium. Bioresour. Technol., 100, 4773–4782.

Hidayat, C. (2017). Utilization of Phytase to Overcome Phytic Acid in Broiler Die. Indonesian Bulletin of Animal and Veterinary Sciences, 26,(2), 057.

Jannah, A. M., Legowo, A. M., Pramono, Y. B., Al-Baarri, A. N., dan Abduh, S. B. M. (2014). Total Bakteri Asam Laktat, pH, Keasaman, Citarasa dan Kesukaan Yogurt Drink dengan Penambahan Ekstrak Buah Belimbing. J. Aplikasi Teknologi Pangan, 3,(2), 7-11.

Juffrie, S., dan Helmyati. (2016). Sinbiotik Evolusi Kesehatan Melalui Saluran Cerna. Gadjah Mada University Press.

Karni, I., Nalurita, I., Pravitri, K. G., Naufali, M. N., dan Meikapasa, N. W. P. (2024). Analisis Struktur Genetik dan Filogenetik Bakteri Lactobacillus Plantarum yang Diisiolasi dari Produk Pangan. Food Scientia: Journal of Food Science and Technology, 4,(1), 72–86

Kerovuo, J., Weymarn, N. V., Poveleinen, M., Auer, S., dan Miasnikov, A. (2000). A New Efficient Expression System for Bacillus and its Application to Production of Recombinant phytase. Biotechnol. Lett., 22, 1311–1317.

Khairunnisah, Bagis, F. A. Z., Andriani, F., Anwar, K., Al Gifari, Z., Rosyidi, A., dan Ali, M. (2022). Isolasi, Identifikasi, Dan Karakterisasi Pediococcus spp. Dan Lactobacillus spp. Dari Saluran Pencernaan Entok (Cairina Moschata) Sebagai Kandidat Probiotik Unggas. Syntax Literate: Jurnal Ilmiah Indonesia, 7,(12), 2541-0849.

Klinberg, T. D., and Budde, B. B. (2006). The Survival and Persistence in The Human Gastrointestinal Tract of Five Potential Probiotic Lactobacilli Consumed as Freeze-Dried Cultures or as probiotic sausage. International Journal of Food Microbiology, 109,(1-2), 157-9.

Lamid, M., Puspaningsih, N. N. T., & Asmarani, O. (2014). Potential of Phytase Enzymes as Biocatalyst for Improved Nutritional Value of Rice Bran for Broiler Feed. J. Appl. Environ. Biol. Sci., 4,(3), 377-380.

Liu, S. Y., Boldb, R. M., Plumsteadb, P. W., and Selle, P. H. (2015). Effects of 500 and 1000 FTU/kg Phytase Supplementation of Maize-Based Diets with Two Tiers of Nutrient Specifications on Performance of Broiler Chickens. Animal Feed Science and Technology, 207, 159-167.

Nuhriawangsa, P. M. (2012). Production of Phytase Powder Results Rebinan and Its Application to Improve Feed Quality and Performance of Broiler Chickens. (Disertasi). UGM. Yogyakarta.

Nurosid, O., Lestari, dan Puji. (2008). Kemampuan Azospirillum sp. JG3 dalam Menghasilkan Lipase pada Medium Campuran Dedak dan Onggok dengan Waktu Inkubasi berbeda. Universitas Soedirman. Purwokerto.

Sajidan, R., Wulandari, E. N., Sari, A. N., Ratriyanto, A., Weldekiros, H., and Greiner, R. (2015). Phytase-producing bacteria from extreme regions in Indonesia. Brazilian Archives of Biology and Technology, 58,(5), 711-717.

Sangadji, I. (2004). Enzim Fitase dan Peranannya dalam Memecah Ikatan Asam Fitat pada Pakan. Makalah Pengantar Falsafah Sains, Program Pasca Sarjana ITB.

Selle, P. H., Ravindran, V., Cadwell, R. A., and Bryden, W. L. (2006). Phytate and Phytase: consequences for protein utilization. Nutrition Reviews, 255-278.

Shamsir, E. (2009). Phytic Acid. Diakses darihttp://ilmupangan.blogspot.com/2009/11/asam-fitat.html.

Sinurat, A. P., Setiadi, P., Purwadaria, T., Dharma, J., dan Haryati, T. (1997). Tingkat penggunaan bungkil kelapa fermentasi dan non fermentasi pada ransum itik petelur. Dalam Kumpulan Hasil-hasil Penelitian APBN Tahun Anggaran 1994/1995. Balai Penelitian Ternak Ciawi. Bogor.

Urbaya, L. N. (2017). Pengaruh Konsentrasi Lactobacillus plantarum terhadap Mutu Tepung Gadung (Discoreahispida Dennst). (Skripsi). Fakultas Teknologi Pangan dan Agroindustri. Universitas Mataram.

Waluyo, L. (2018). Mikrobiologi umum. UMM Press.

Wang, L., Yang, Y., Cai, B., Cao, P., Yang, M., and Chen, Y. (2014). Coexpression and Secretion of Endoglucanase and Phytase Genes in Lactobacillus reuteri. Intj. Mol. Sci., 15, 12842-12860.

Wibawa, A. A. P., Wirawan, I. W., dan Partama, I. B. G. (2015). Peningkatan Nilai Nutrisi Dedak Padi Sebagai Pakan Itik Melalui Biofermentasi Dengan Khamir. Majalah Ilmiah Peternakan, 18,(1), 11-16.

Winarno, F. G. (2021). Pangan Pelancar Aliran Darah dan Diet alkali. PT Gramedia Pustaka Utama.

Yunita, M., Hedrawan, Y., dan Yulianingsih, R. (2015). Analisis Kuantitatif Mikrobiologi Pada Makanan Penerbangan (Aerofood ACS) Garuda Indonesia berdasarkan TPC (Total Plate Count) dengan Metode Pour Plate. Jurnal Keteknikan Pertanian Tropis dan Biosistem, 3,(3), 237-248.

Zurmiati, Mahata, M. E., Abbas, M. H., dan Wizna. (2014). Aplikasi Probiotik untuk Ternak Itik. Jurnal Peternakan Indonesia, 16,(2), 134-144.

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Published

25-06-2025

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