Skrening Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Broiler sebagai Kandidat Probiotik untuk Unggas

Abstract

The use of probiotics is increasingly popular as an alternative replacement to antibiotic uses in stimulating health
and growth of broiler chickens. The use of antibiotics may disturb the balance of the gut microbiota and play a
significant role in the emerging of antibiotic resistance pathogens. Thus, the objective of this study was to isolate
and screen lactic acid bacteria (LAB) from the intestine of broiler chickens as probiotic candidates. LAB were
initially screened by phenotypic assay. Furthermore, those strains which belonged to LAB were screened for
inhibitory activity against E. coli. Results showed that a total of 4 isolates were considered as members of lactic
acid bacteria indicating by the results of phenotypic assays: gram positive, catalase and oxidase negative, and able
to perform carbohydrate fermentation. Out of the 4 isolates, only one isolate showed an inhibitory activity against
the targeted pathogen. Further research is still needed to confirm the potential strain for probiotic in poultry.